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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: hubbcut on March 06, 2024, 09:48:35 AM

Title: Making a stock solution in DMSO
Post by: hubbcut on March 06, 2024, 09:48:35 AM
Hi! What does it mean to make a 1M stock solution of Nile Red in DMSO? I've never worked with either reagent before and unsure of the calculations to make this. How do I go about it?
Title: Re: Making a stock solution in DMSO
Post by: Borek on March 06, 2024, 11:37:45 AM
Don't get sidetracked by the substances involved, it is a just a solute and solvent. Concentration is number of moles of Nile Red per volume of the solution, nothing more fancy.
Title: Re: Making a stock solution in DMSO
Post by: hubbcut on March 07, 2024, 04:52:10 AM
Quote from: Borek on March 06, 2024, 11:37:45 AM
Don't get sidetracked by the substances involved, it is a just a solute and solvent. Concentration is number of moles of Nile Red per volume of the solution, nothing more fancy.

Yes but I'm unsure how to start. I don't know how much DMSO I'm supposed to dissolve into water. The paper I'm using as a basis for my study only says to make a 1M stock solution of Nile Red, but doesn't say how much DMSO is needed in the final concentration.
Title: Re: Making a stock solution in DMSO
Post by: Hunter2 on March 07, 2024, 05:07:30 AM
I think there is no water involved. The stock solution uses DMSO as the solvent.
Title: Re: Making a stock solution in DMSO
Post by: Babcock_Hall on March 07, 2024, 01:40:05 PM
https://docs.aatbio.com/products/protocol/22190.pdf

I wonder whether you might mean a 1 mM stock solution in DMSO.  I am not sure what the solubility limit is.
Title: Re: Making a stock solution in DMSO
Post by: Hunter2 on March 07, 2024, 03:44:21 PM
In the TDS its clearly written to use  anhydrous DMSO.
Title: Re: Making a stock solution in DMSO
Post by: rjb on March 07, 2024, 07:00:16 PM
Quote from: Babcock_Hall on March 07, 2024, 01:40:05 PM
https://docs.aatbio.com/products/protocol/22190.pdf

I wonder whether you might mean a 1 mM stock solution in DMSO.  I am not sure what the solubility limit is.

This takes me back! I think you're right... According to my old scribbled notes, it's soluble in DMSO up to about 10mM. With current prices a 1M solution would be quite an expensive undertaking!

R