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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: spicooli on October 02, 2007, 07:40:01 AM

Title: calculate volume of buffer in reaction mixture
Post by: spicooli on October 02, 2007, 07:40:01 AM

Hi guys,

I have this urgent problem.
How do you calculate the volume of potassium phosphate buffer to add in a 1ml reaction mixture? The reaction mixture must contain 100mM potassium phosphate buffer, 0.3mM H202, 0.5mM ascorbate and the enzyme extract.

I have 1L of 100mM potassium phosphate buffer. I want to know how much to add into the reaction mixture.

Hope you guys can help me. Thanks a lot!

Title: Re: calculate volume of buffer in reaction mixture
Post by: AWK on October 02, 2007, 08:49:17 AM

Any addition to 100mM buffer dilute it. You should prepare other stock solution (eg 200 mM) or use 100 mM buffer and neglect its dilution.