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Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: shrikantvarma on July 29, 2010, 10:30:20 AM

Title: no ionization in LC-MS
Post by: shrikantvarma on July 29, 2010, 10:30:20 AM

while doing LC-MS my compound not get ionize,so i not got my M.W.,what can i do to get it ionize so i get M.W. of my compound??

Title: Re: no ionization in LC-MS
Post by: MOTOBALL on July 29, 2010, 09:09:17 PM

Difficult to help without the following information,

1.   Name of compound ??

2.   HPLC mobile phase ?

3.   Ionization mode +ve or -ve ?

Title: Re: no ionization in LC-MS
Post by: Mesogen on August 24, 2010, 11:16:01 AM

Is it that the compound is not being ionised or that the compound is being completely fragmented (and therefore you don't see the main ion but see the fragmentation pattern?)

If it's the latter try using a softer ionisation technique...