Chemical Forums
Chemistry Forums for Students => Organic Chemistry Forum => Topic started by: mahayoub on November 14, 2012, 01:10:46 PM
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Hello everyone :)
I am going to work on protein hydrolysis in the seed dates, but I search many times about how to pack the gel filtration chromatography and I couldnt find a good answer. I need this for my proposal research. I want to know the experiment and how to pack the column in order to fraction proteins according to their sizes.
Thanks
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Shall we write the research proposal for you? It would save time.
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You may find a good laboratory text, or you might find a simple handbook around the lab--vendors create these, to teach people how to use the stuff they sell. Like discodermolide: has alluded to, you have asked too general a question. What, in your vast array of search results, have you read, that you don't understand, or are unsure of which options to take?
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Shall we write the research proposal for you? It would save time.
Thanks for helping
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You may find a good laboratory text, or you might find a simple handbook around the lab--vendors create these, to teach people how to use the stuff they sell. Like discodermolide: has alluded to, you have asked too general a question. What, in your vast array of search results, have you read, that you don't understand, or are unsure of which options to take?
Yes, i am unsure which options I have to take. I just want to know the basic method to prepare the column according to my topic. Thank you :)
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I suggest you read pages 125-133 in Fundamental Laboratory Approaches for Biochemistry and Biotechnology, by Ninfa, Ballou, and Benore, 2nd edition, Wiley. You might also try Pharmacia's book "Gel Filtration: Principles and Methods", which is available as a free pdf download from Amersham Biosciences.
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I suggest you read pages 125-133 in Fundamental Laboratory Approaches for Biochemistry and Biotechnology, by Ninfa, Ballou, and Benore, 2nd edition, Wiley. You might also try Pharmacia's book "Gel Filtration: Principles and Methods", which is available as a free pdf download from Amersham Biosciences.
Thank you. The books are very useful. :)