Chemical Forums
Chemistry Forums for Students => Analytical Chemistry Forum => Topic started by: alchmemist54656 on May 15, 2013, 06:55:01 PM
-
I'm starting out as an undergraduate in a research lab and will soon be doing HPLC over the summer. However, most of the chemists in my lab have limited experience with HPLC. How can I get started with using it. What are some good books that will help me learn chromotography, specifically HPLC?
-
I would start with the very basics of HPLC theory from a general Analytical Chemistry textbook. However, this will really teach you very little about proper day to day use of modern instruments. That's something that really doesn't go in a textbook. If you've got some money to spend, LC Resources gives very in-depth courses, in-house, or at an event they host, or (nowadays more and more) real-time online. But the courses are very through, and a little expensive. Maybe the beat way to learn what's expected of you from this position is to find the instrument expert -- there must be at least one -- and ask if you can shadow them as they take the instruments through their paces.
-
If you want just to use the HPLC, just look at look at HPLC vendors' website.
Agilent, Waters, Dionex, and Shimadzu are big HPLC manufacturers.
Agilent has a very good primer on HPLC
http://www.chem.agilent.com/Library/primers/Public/5989-6639EN.pdf
Also look at Waters' website for basic introduction.
http://www.waters.com/waters/en_US/HPLC---High-Performance-Liquid-Chromatography/nav.htm?locale=en_US&cid=10048919