One molecule has HC triple bonded to a carbon that is single bonded on the other side to another carbon with 3 single bonds to 3 CH3 molecules. The second molecule has a carbon atom single bonded to 3 H atoms and another carbon atom which is single bonded to 3 CH3 molecules. Is the single bond between the C atom triple bonded to HC and the C atom single bonded to 3 CH3 molecules shorter and stronger than the single bond between the C bonded to two sp3 carbon atoms?

Can you make another batch? It does not look expensive to make?

What follows (from Barni F et al. 2007) is the recipe for the Weber formulation of luminol.

Weigh out 8 g of sodium hydroxide and completely dissolve them in 0.5 L of deionized water to obtain a 0.4 N solution (stock solution A).

Measure 10 mL of 30 % hydrogen peroxide and add them to 0.49 L of deionized water to obtain a 0.176 M solution (stock solution B).

Weigh out 0.354 g of luminol and completely dissolve them in 0.0625 L of 0.4 N sodium hydroxide solution to obtain a final volume of 0.5 L (0.004 M) (stock solution C).

Store the three stock solution in glass or plastic containers at 4° C, away from direct light.

Prepare the test solution by mixing 0,01 L of each of the three stock solutions to 0.07 L. of deionized water to obtain 0.1 L. of final working solution.

Decant solution into a vaporizer or sprayer and use immediately.


Tobe SS et al., J. Forensic Sci. 2007 Jan;52(1):102-9.  doi: 10.1111/j.1556-4029.2006.00324.x.
Regarding how to perform a test, I found a discussion that might be helpful.
"The six different [color-change] reagents were tested against saliva, semen, potato, tomato, tomato sauce, tomato sauce with meat, red onion, red kidney bean, horseradish, 0.1 M ascorbic acid, 5% bleach, 10% cupric sulfate, 10% ferric sulfate, and 10% nickel chloride.  For each of the presumptive reagents tested, a large piece of filter paper (approximately 100 cm²) was exposed to each of the substances being tested in 25 separate sample stains. These were allowed to dry for a minimum of 18 h. Each of the pieces of filter paper, and subsequent stains, were then tested with their corresponding reagent to see whether the substance caused a reaction."

100% polyester white fabric is a slightly transparent fabric. Does this fabric provide good protection to prevent plastic and rubber from drying out and breaking in electronic devices?

Is the sunlight that dries out plastic and rubber in electronic devices any light, even indirect light that is deflected and enters homes?

I see! I've also been told by others that leaving the luminol out in the fume hood in a container was the issue as the luminol decomposes in light. Thank you your insights.

Just to clear up some uncertainties, here are some of volumes and molarities I used:
10mL NaOH (1M)
0.2g Luminol
115ml Water
10mL H2O2 (3%)
0.25mL CuSO4 (1M)

I just wanted to clarify, by combining the NaOH and the luminol solution (hydrazide) it caused hydrolysis due to becoming basic and thus the solution became bad?

And for the future, should I just mix the Luminol solution and the H2O2 and then the catalyst at the moment of the experiment?

I was actually planning on changing the concentration of the H2O2 and determining the effect it has on the intensity of the reaction. I was going to do 10 different concentrations for 5 trials each, would this mean I should prepare the luminol solution for each trial at the moment of doing it?