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71
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by mana on March 07, 2025, 06:48:54 AM »
First check, did you get any alcohol, if you dont use current?
no I didn't get any alcohol, and yes used current

Why do you think to get alcohol with current, if you dont get it without.
The reaction has to run without first. If this works, then you can accelerate with current.
yes you are right, I haven't thought about this, I have tested this reaction without electric field many times with different additives such as PBS buffer, yeast extract, ammonium sulfate or different amounts of glucose and yeast but in all cases we didn't achieved ethanol, it's better to say we achieved acetic acid instead, because the reaction mixture smelled like vinegar, I don't know, but I assume that this is because oxygen presence in our mixture reaction, and unfortunately we can't omit it because we don't have nitrogen gas or any other inert gases to inject to reaction mixture for oxygen removing, is there any way in your point of view to solve this problem, for example is there any yeast which can produce alcohol even in presence of oxygen (instead of saccharomyces cerevisiae) or etc.
72
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by Hunter2 on March 06, 2025, 03:02:43 PM »
True. He set 15,6 V

Jones Reagent and a alcoholmeter was used to check for  Ethanol.

Quote
After this time we tried to measure the alcohol percentage with an alcoholmeter, and it showed nothing, we tried Jones reagent to check and it didn’t show any green color either, again 1 gram of fresh yeast was added to this solution and the reaction was kept in mentioned situation but it didn’t change the results.
73
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by Borek on March 06, 2025, 02:05:10 PM »
"Electrostatic" means no current, just an electric field.

20 g of glucose per 50 mL of water sounds way too high, that's not what the original paper used.

How do you check for the ethanol presence?
74
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by Hunter2 on March 06, 2025, 12:39:25 PM »
First check, did you get any alcohol, if you dont use current?
no I didn't get any alcohol, and yes used current

Why do you think to get alcohol with current, if you dont get it without.
The reaction has to run without first. If this works, then you can accelarate with current.
75
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by mana on March 06, 2025, 12:34:19 PM »
and this is the picture of the situation that has mentioned in the paper
76
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by mana on March 06, 2025, 12:32:41 PM »
First check, did you get any alcohol, if you dont use current?
no I didn't get any alcohol, and yes used current
77
Organic Chemistry Forum / Re: Achieving ethanol from D-glucose problem
« Last post by Hunter2 on March 06, 2025, 12:27:25 PM »
First check, did you get any alcohol, if you dont use current?
What kind of bakery yeast did you use? Bakery yeast is normally  used to make bread. Not for production of alcohol. Better is get brewers yeast or nutritional yeast.
78
Organic Chemistry Forum / Achieving ethanol from D-glucose problem
« Last post by mana on March 06, 2025, 12:23:29 PM »
hi all
I have read this amazing paper entitled : Enhanced ethanol production via electrostatically accelerated fermentation of glucose using saccharomyces cerevisiae.

My students and I have been trying to produce ethanol from D-glucose with different protocols many times, but our efforts have not been successful yet. We have tried the protocol mentioned in your interesting paper with some changes due to our lack of facilities, and we didn’t achieve ethanol. A picture of our procedure has been attached.

In this condition, 20 grams of D- glucose was dissolved in 50 ml of distilled water, and 1 gram of bakery yeast was added to the solution, we put a carbon electrode connected to positive current and another carbon electrode which was stuck to the beaker wall connected to negative current (proposed by chat GPT) in the circuit, the voltage was set on 15.6 v and the reaction was kept on a radiator which temperature was about 33oC for 24 hours, and the beaker was closed by using parafilm strip.  After this time we tried to measure the alcohol percentage with an alcoholmeter, and it showed nothing, we tried Jones reagent to check and it didn’t show any green color either, again 1 gram of fresh yeast was added to this solution and the reaction was kept in mentioned situation but it didn’t change the results.

I know our procedure is very different from what has mentioned in the paper, but unfortunately, we can’t provide the negative electrode and nitrogen gas and some other criteria as mentioned in the paper. I would be very grateful if you could tell us what we can do with our limitations to use this procedure and achieve ethanol.
thank you in advance for your help
79
Organic Chemistry Forum / analysis of odor in liquid nettle manure?
« Last post by mistryous on March 06, 2025, 07:34:21 AM »
Can someone please give me at least some more in-depth information on why the liquid manure of the nettle plant has that odor of sewage to it?
I cannot find anything valuable except for a hint that the odor may mostly result from the fermentation and decomposition of histamine, serotonine, etc. - as present in the stuff that stings you.
Well, it would explain why the odor is so special to this plant's liquid manure, but I don't know.
I would like to know in more exact terms.

What would be the resulting odorants, then? Some indole and amine molecules of some kind, maybe? But what exactly?
Is there anything existent that's close to an analysis to this problem? An article incl. a GCMS analysis of the odorants would be perfect but I doubt that it exists? I couldn't find anything valuable.
???
80
Organic Chemistry Forum / Re: Boronic acid for suzuki-myura coupling
« Last post by dani on March 06, 2025, 05:42:16 AM »
Hi , thanks for your answer, I will buy the reagent, both boronic acid and pinacol boronic ester of phenilacetic acid are avalable.
so you think that my approach (coumarin formation 2 top image) could work and the final product could be used in a suzuki miyura coupling?
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