[Mater1991]

Hello everyone:
I have some graphene oxide(GO) with  carboxylic acid functional groups that I wanted to couple to aminated graphene oxide(AGO).In order to activate carboxylic acids of graphene oxide that I put EDC/NHS to the solution.I have the question that how high the temperature of experiment EDC/NHS will fail,I want to get a fast and feasible reaction rate.
So,what's your temperature in EDC/NHS experiment,I wanted to get your opinions first. Any advice appreciated!

Some more info:
Solvent：DMF
Now the experimental temperature is 185℉/85℃

[wildfyr]

I've always done it at room temperature overnight. I feel like the only time I've seen raised temps for it was to better solvate one of the reagents.

How did it go at 85°C?

[hollytara]

My experience is at room temperature also - and usually in water.  Can you use a different coupling agent in DMF?

[wildfyr]

Do it in acetonitrile. The DCU byproduct is poorly soluble in it, so the workup is easier.