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Topic: Help me to messure the melting curve of nucleic acid analogues  (Read 5316 times)

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Offline bingchen

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Help me to messure the melting curve of nucleic acid analogues
« on: October 04, 2006, 03:26:50 PM »
I want to messure the melting curve of nucleic acid analogues containing uracil and adenine as base. Can you tell me what kinds of buffer solution I should use. Does anyone have experience of observation of the formation of base pairs by NMR. Thanks in advance! :)               
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Offline Yggdrasil

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Re: Help me to messure the melting curve of nucleic acid analogues
« Reply #1 on: October 04, 2006, 06:38:25 PM »
Why are you using NMR?  Can't you just look at the change in absorbance at 260nm?
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Offline bingchen

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Re: Help me to messure the melting curve of nucleic acid analogues
« Reply #2 on: October 05, 2006, 04:43:41 PM »
I can not see the change of absorbance in UV when I use water as solvent. I hope to see the chemical shift of proton in N-H in the base pairs moves in NMR.
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