[Soulja]

Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

[jeffmoonchop]

How about water?

[Orcio_87]

Water may not help if the methanol denatured the protein.

[Corribus]

What are you hoping that UV-Vis will tell you?

[Soulja]

Hey all,

I am working on a project involving an extraction of an allergen protein. I was able to extract it from the raw source, and it is currently in a freezer. My work requires me to analyze it via several methods of analysis. I am having trouble running my protein via UV-Vis; the main problem being that my protein just doesn't seem to be soluble in any solvents I use. So far I've tried acetone and methanol (which was used in the extraction process), but i've had no luck with sonication and stirring. Does anyone have any potential suggestions?

THanks.

[billnotgatez]

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[Babcock_Hall]

Buffered water is the first thing that I can think of.  Acetone is likely to precipitate a protein.

[morphism]

Hi! What kind of allergen protein?