[gaschroking]

Good day,

I usually do hydrocarbon extraction from my culture using an equal amount of dichloromethane  as an extraction solvent. Previously, i had been using 10 ml of culture. So, extraction using 10 ml of dichloromethane is very manageable. Now, I started to set-up a 30 ml culture. Would it be Ok to do extraction using 10 ml of dichloromethane? In solvent extraction, is it a protocol to do extraction using the same amount of solvent?

Could you help me on this matters...


Many thanks

[Borek]

Usual procedure is to use smaller volume of the solvent, but to do extraction in three steps. This way extraction goes much closer to completion.