[ROSETTA]

Hi, can the carbonyl groups of caffeine act as weak hydrogen bond acceptors creating interactions with silanol groups in reversed phase chromatography
Thanks
Rosie

[Arkcon]

Caffeine is a common testing, or training analyte for HPLC.  Google should clog your day with data if you did the search.  Depending on the quality of the media, you may or may not see bad peak shape due silanol interaction.  Virtually any molecule can be affected by a poor quality column, of the technology that was available back in the pre-history of HPLC (when I started, back in the '80's)