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Topic: how do i prepare this buffer?  (Read 6547 times)

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Offline coquim

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how do i prepare this buffer?
« on: February 21, 2008, 12:49:24 PM »
Sodium phosphate 50mM but to ph=2,5
i couldnĀ“t find anyting in books and on the net.
Could anyone please help me with this?
Is it possible to adjust the ph with phosphoric acid?
Thanks!!!!!!

Offline Borek

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Re: how do i prepare this buffer?
« Reply #1 on: February 21, 2008, 12:58:58 PM »
Is it possible to adjust the ph with phosphoric acid?

Exactly - and that's what you are supposed to do.
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Offline Arkcon

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Re: how do i prepare this buffer?
« Reply #2 on: February 21, 2008, 01:01:22 PM »
That's one way of doing it, 'tho some people don't care for that method much.  You can also prepare it from the correct mixture of sodium phosphate monobasic and dibasic.  I think there may be some source for a buffer prep program, I just can't recall where ... if only someone could provide a buddy for making buffers ...
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline agrobert

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In the realm of scientific observation, luck is only granted to those who are prepared. -Louis Pasteur

Offline Borek

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Re: how do i prepare this buffer?
« Reply #4 on: February 21, 2008, 01:42:12 PM »
You can also prepare it from the correct mixture of sodium phosphate monobasic and dibasic.

Better think it over... pH 2.5 is too low for that.

Quote
I think there may be some source for a buffer prep program, I just can't recall where ... if only someone could provide a buddy for making buffers ...

Buddy... could be ChemBuddy? ;)

Wanna be a betatester? Release date not yet set, hopefully about a month or two from now. Betatests will start earlier.

Note: there are several programs that pretend to be able to calculate buffer. Those that I have seen are based on misunderstood Henderson-Hasselbalch equation, so they will tell you how to prepare 0.01M pH 2.33 buffer from chloroacetic acid - when pure chloroacetic acid of this concentration has pH around 2.5. They will also not calculate how to prepare citrate/phosphate buffer, as it is not described by HH.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info

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