[MARIANA]

Hi.
My name is Mariana and I work as an R&D Chemist within a pharmaceutical company. I would really appreciate if you can give me some suggestions for the problem I have.
I have a brand new job and my first project is to extract the PSGAG (Polysulfated Glycosaminoglycan) from a canine tissue (intramuscular the PSGAG injection was administrated to dogs and I have to quantitate, or at least show qualitatively that PSGAG is present). I developed a HPLC method using size-exclusion chromatography column and 20mg/L Calcium propionate (aqueous) as mobile phase that seems to work just fine with the PSGAG reference standard material (dissolved in the mobile phase). But my problem is how do I extract the PSGAG from the tissue and bring it into a liquid form so I can test it. I found in some journal that they use some enzymes? Is it any other easier way to do it?
Thank you.

[Arkcon]

I would suggest starting with the published method and later seeing if you can modify it.  I realize that's time consuming, but it at least gives you a baseline.  You can always keep looking for methods, searching for similar compounds, or very different tissues with the same analyte for other methods.  I'm thinking animal tissue may well require enzymes to break down muscle tissue, if that is the matrix you're working with.  Plant tissue responds well to freezing and detergents, but that's a totally different matrix.