Dear Jessy;
The answer for the first Question lies already in the second:
The “Elution Mixture”, or Water, transports something till to the top, but not all kinds with the same “Migration Speed”.
So what will be the consequence of that?
If you have answered the first Question:
What will be the “benefit” if also the slowest migratiting “Spot” reachs the “target” (the top)?
For what in this case will you have done the whole work?, ─ For Nothing!!
I hope the last will never happen to you, but I have been of help .
Good Luck!
ARGOS++