Not sure about that kind of bio-fuel ( will learn more about that in the next month or so,)
Run my own car on biofuel (rapeseed base) but dont make my own, but know of several people that do. relatively easily with "recipes" easily available on the net.
But running a two-stroke Goped, Why? if your thinking of lowering your carbon footprint that way, you'd be better off not eating beef. A cow produces more greenhouse gases per annum out of its backside than the average family car.