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Topic: Concentration of compound  (Read 3993 times)

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Offline ST_08_09

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Concentration of compound
« on: May 31, 2008, 09:45:20 AM »
A compound with a molecular weight of 292.16 was dissolved in a 5 ml volumetric flask.
Then withdrawn 1ml aliquot,placed in a 10 ml volumetric flask and diluted to the mark.The absorbency at 340 nm was 0.427 in a 1cm cuvet. The molar absorptivity for this compound at 340 nm is ε=6130
(M Inverse) (CM Inverse)
The Problem is (1)How can i approach to calculate the concentration of compound in the cuvet.
Secondly(2)What was the concentration of compound in the 5 ml flask ?
Third (3) How many milligrams of compound were used to make the 5 ml solution ?

Thanks in advance

Offline Yggdrasil

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Re: Concentration of compound
« Reply #1 on: May 31, 2008, 11:20:29 AM »
Look up the Beer-Lambert Law.

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