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Topic: What means 50% Proline?  (Read 6133 times)

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Offline ali

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What means 50% Proline?
« on: August 14, 2009, 12:01:51 PM »
Hi,

I have a recipe for tissue culture medium and this recipe is asking for 50% proline it does not state how much (mg, g etc,)?  I do not understand it?
I tried to look for some answers before with no success.

Could someone please help me?

Thank you.

Offline sjb

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Re: What means 50% Proline?
« Reply #1 on: August 14, 2009, 02:48:22 PM »
What's the full recipe? Without much context, anything could be the answer, to be honest.

Offline ali

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Re: What means 50% Proline?
« Reply #2 on: August 14, 2009, 04:53:58 PM »
Hi,

It is just simple MS medium supplemented with 1.0 mg/l 2,4-D,
0.5 mg/l BAP and 50% proline.

Hope that gives some idea.

Thanks
Regards,

Offline JGK

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Re: What means 50% Proline?
« Reply #3 on: August 14, 2009, 07:26:35 PM »
the unit should be either % w/w (weight/weight), % w/v (weight/volume) or % v/v (volume/volume) and is in terms of g/100 g (w/w), g/100 mL (for w/v) or mL/100 mL (for v/v)

If the proline is a solid then a 50% w/v will be 50g proline in 100 mL of solvent.
Experience is something you don't get until just after you need it.

Offline ali

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Re: What means 50% Proline?
« Reply #4 on: August 16, 2009, 05:26:15 AM »
Thank you very much for your *delete me*

Offline ali

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Re: What means 50% Proline?
« Reply #5 on: August 16, 2009, 05:30:25 AM »
I mean, thank you for your kind replay!

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