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Topic: enzyme kinetic  (Read 3127 times)

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Offline naj4279

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enzyme kinetic
« on: October 11, 2009, 12:04:26 PM »
Hi,

I run an enzyme kinetic assay and plotted the LB curve. My y-axis is 1/v in (absorbance/time) unit. How do i change this into ug to give a reasonable Vmax value?
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Offline Yggdrasil

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Re: enzyme kinetic
« Reply #1 on: October 11, 2009, 06:09:31 PM »
You need to know the extinction coefficient of the substance whose absorbance you are measuring.  Try constructing a standard curve to determine this value.
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