[gggggggggg]

so, i have plotted my 1) initial velocity (v) against substrate concentration ,S.
                               2) Lineweaver-burk plot)
                               3)Eadie-Hofstee plot
                               4)Woolfe plot
And was asked to calculate the michaelis constant (Km) and the maximum velocity (Vmax) from each plot.

I understand what each graph demonstrates in terms of for eg :

1) Shows Vmax and Km  ( This one i can do)

2)              o y intercept = 1 / Vmax     (This one, i dont know how to get Km and Vmax from all this information)
                o  gradient = Km / Vmax
                o   x intercept = -1/ Km

3)
          o y intercept = Vmax               (This one , i know the Vmax, and i think i know the Km, its just changing signs right?)
          o gradient = -Km
          o x intercept = Vmax / Km

4)

          o y intercept = Km / Vmax    (This one i know the Km value [its just the opposite sign right?] , but dont know gow to get Vmax)
          o gradient = 1 / Vmax
          o x intercept = -Km

If you could help me in the areas i have doubts, it would be appreciated.
Thanks

[sjb]

2)              o y intercept = 1 / Vmax     (This one, i dont know how to get Km and Vmax from all this information)
                o  gradient = Km / Vmax
                o   x intercept = -1/ Km

Simple manipulations, so for instance in this one if you have the y-intercept, then its reciprocal is V_max, similarly the x-intercept has a reciprocal of -K_m..?

S

[gggggggggg]

2)              o y intercept = 1 / Vmax     (This one, i dont know how to get Km and Vmax from all this information)
                o  gradient = Km / Vmax
                o   x intercept = -1/ Km

Simple manipulations, so for instance in this one if you have the y-intercept, then its reciprocal is V_max, similarly the x-intercept has a reciprocal of -K_m..?

S

If i have -Km , is it just a change of sign to get Km? Will that affect my end results?