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Topic: disulfide bonds  (Read 6841 times)

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stasia222

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disulfide bonds
« on: September 29, 2005, 04:57:39 PM »
Hi, I need to know how or what, other than using Glutathione, can break disulfide bonds at a neutral pH.
Thanks
Stacey
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Offline lemonoman

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Re:disulfide bonds
« Reply #1 on: October 01, 2005, 02:56:38 PM »
To break the disulfide bond of cystine and form a thiol at each sulfur, they suggest added exces of 2-mercaptoethanol.

See this VERY general mechanism:



from http://www.biochemj.org/bj/357/0343/bj3570343.htm
« Last Edit: October 01, 2005, 03:00:00 PM by lemonoman »
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