[dhkwak]

Hey guys,

I had recently implemented CD of bovine serum albumin (BSA) and had expected negative minimum at about 208 and 222 nm (as it is predominantly of alpha helical secondary structure); unfortunately, I was only able to obtain a single negative minimum at approximately 222 nm. I am wondering if this may be due to either a buffer issue or low concentration. I am using approximately 100 mM PBS with a protein concentration of 1 uM (a total of about 70 ug in 1 mL). Any help would be greatly appreciated! Thanks