[luysion]

hey,got this Q in my textbook with no answer, i really want to work through it but i dont know whether or not the A i get is right or wrong..

A 24 hour urine sample is collected and analysed for albumin using the BCG assay. The total volume of urine is 0.8L

0.9 mL of a 1 in 2 dilution is analysed spectrophotometrically and the absorbance is 0.2. This value is in the linear part of a standrd curve generated for this assay. From the standard curve the absorbance of 0.1mg of albumin is 0.1 and 0.5 mg of albumin is 0.5.

Using a molecular weight for albumin of 66000, how many μmol of albumin are present in the original urine sample. (only enter the number and use no more than 2 decimal places)

its a little complex for me
appreciate the help yea

[Borek]

Do it step by step.

What is concentration of albumin in diluted sample?

In undiluted sample?

What is mass of albumin in the original sample?

How many moles is it?