So i have to do a quench calibration curve.
0.32ml of H-hexadecane ( specific radioactivity 1.5x 107 d.p.m/g, specific gravity, P20, = 0.664g/ml) was added to 250 ml with toluene at 20o. 1.0 ml of standard toluene solution was added to 4 ml of scintillant in each vials 1-12, together with progressively increasing amounts water, which quenches the counting process.
I have to plot ESR ( external standard ratio) against the efficiency of counting (%) for this scintillation counting system.
I have my values of ESR and Cpm in my table and i know that i need to use % = (Cpm X 100) / dmp. But i'm having trouble with the dilution factor and i don't know why specific gravity is given. Don't i just assume that if 0.32 ml of H-hexadecane is 1.5x 107 d.p.m/g then by taking 1 ml of the standard solution i get 4.68 x 10 7 or do i need to use the specific gravity to get a from dmp/g to dmp.
Thank you for your consideration
J.J