[mehc]

Facing some confusion in GC quantification.

Suppose we prepare a plant extract and analyze it by GC for some analytes.

Use of standard (in GC method) gives concentration of one analyte in mg/ml. How can we convert it in mg/g of plant material.

Definitely, we will use weights of material used and extracted in the course of  method, but i am searching for exact equation.

Really need some help in this regard.

Thanks

[Stepan]

W(mg/g)=C(mg/mL)*V(mL)/Mplant(g)
W- analyte content in plant
C-concentration in extract
V-extract volume
Mplant- Mass of plant taken for extraction.

[mehc]

W(mg/g)=C(mg/mL)*V(mL)/Mplant(g)
W- analyte content in plant
C-concentration in extract
V-extract volume
Mplant- Mass of plant taken for extraction.

Thanks a lot,
In my opinion, this equation is more suited for hplc quantification. In gc, concentration of sample injected should also taken into consideration. In one case, if we injected sample from 20 mg/ml, and in other case 30mg/ml, it will affect the quantification.

[Stepan]

This very very basic formula  has nothing to do with analytical method you use. It assumes that you know how to measure concentration in your extract (C). How you do it - it is up to you.

PS. I did not get your example with 20 and 30 mg/ml and how GC is different from LC, sorry