[HB]

I have an extract from plant origin that contain a protein component and polysaccharide component and I need to know the chemical structure of both products. what are the steps need to be done?

[MOTOBALL]

Does the extract contain a protein component mixed with a polysaccharide component, or is it a covalently linked Prot-Carb, i.e. a glycoprotein ??

In any case,

1) the extract needs to be purified to homogeneity (not a trivial task),

2) the protein requires (at a minimum) MW detmn; aminoacid analysis (AAA); N-terminal sequencing (Edman degradation); cyanogen bromide cleavage at Met residues to generate polypeptides; peptide mapping of these fragments and also of trypsin/chymotrypsin digests; disulfide bond location. 

This is a labor and instrumentation-intensive process (i.e. $$$$$).

3) the polysaccharide needs MW detmn; quantitative monosaccharide analysis; sequence analysis; linkage analysis; alpha/beta anomeric detmn.; point(s) of attachment to protein.

This is another $$$$ process.