[mahayoub]

Hello everyone   

I am going to work on protein hydrolysis in the seed dates, but I search many times about how to pack the gel filtration chromatography and I couldnt find a good answer. I need this for my proposal research. I want to know the experiment and how to pack the column in order to fraction proteins according to their sizes.

Thanks

[discodermolide]

Shall we write the research proposal for you? It would save time.

[Arkcon]

You may find a good laboratory text, or you might find a simple handbook around the lab--vendors create these, to teach people how to use the stuff they sell.  Like discodermolide: has alluded to, you have asked too general a question.  What, in your vast array of search results, have you read, that you don't understand, or are unsure of which options to take?

[mahayoub]

Shall we write the research proposal for you? It would save time.

Thanks for helping

[mahayoub]

You may find a good laboratory text, or you might find a simple handbook around the lab--vendors create these, to teach people how to use the stuff they sell.  Like discodermolide: has alluded to, you have asked too general a question.  What, in your vast array of search results, have you read, that you don't understand, or are unsure of which options to take?

Yes, i am unsure which options I have to take. I just want to know the basic method to prepare the column according to my topic. Thank you 

[Babcock_Hall]

I suggest you read pages 125-133 in Fundamental Laboratory Approaches for Biochemistry and Biotechnology, by Ninfa, Ballou, and Benore, 2nd edition, Wiley.  You might also try Pharmacia's book "Gel Filtration:  Principles and Methods", which is available as a free pdf download from Amersham Biosciences.

[mahayoub]

I suggest you read pages 125-133 in Fundamental Laboratory Approaches for Biochemistry and Biotechnology, by Ninfa, Ballou, and Benore, 2nd edition, Wiley.  You might also try Pharmacia's book "Gel Filtration:  Principles and Methods", which is available as a free pdf download from Amersham Biosciences.

Thank you. The books are very useful.