[peptideismylife]

Hey,

I am willing to carry out a Boc deprotection in my peptide, which has the follow sequence:

Fmoc-Lys(Boc)-Ser(tBu)-Ala-Gly-Ala-Wang Resin

Usually to deprotect Boc I employ TFA/TIPS, but then I will also cleave the Resin...and it's something that I don't want it...I will only cleave the Boc group in the Lysine, and not tBu as well as Fmoc and the resin...so I want to get....:

Fmoc-Lys-Ser(tBu)-Ala-Gly-Ala-Wang Resin

Any suggestion? lower % of TFA?

Thanks!

[Honclbrif]

http://www.amazon.com/Greenes-Protective-Groups-Organic-Synthesis/dp/0471697540/ref=sr_1_1?ie=UTF8&qid=1306444905&sr=8-1

[Dan]

This may be of interest:

http://www.sciencedirect.com/science/article/pii/S0040403903009936

[Åke]

For N-Boc deprotection, you might be able to get away with TBAF¹. I have no experience with the solid-phase synthesis but I guess the resin should be fine.

1. Routier, S.; Sauge, L.; Ayerbe, N.; Coudert, G.; Merour, J.-Y. A mild and selective method for N-Boc deprotection. Tetrahedron Lett. 2002, 43, 589−591. http://www.sciencedirect.com/science/article/pii/S0040403901022250

Regards,

Vedran