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Topic: HELP with determine an unknown amino acid  (Read 3367 times)

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Offline IS300bklyn

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HELP with determine an unknown amino acid
« on: September 17, 2012, 02:06:14 PM »


I can't seem to determine what type of amino acid these both graphs are, both graphs are titrated from the same unknown.
Thanks

Offline Babcock_Hall

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Re: HELP with determine an unknown amino acid
« Reply #1 on: September 17, 2012, 02:48:39 PM »
The two sets of two pKa values are different.  I don't see how this can be the same amino acid.

Offline fledarmus

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Re: HELP with determine an unknown amino acid
« Reply #2 on: September 17, 2012, 03:07:26 PM »
Can you limit your choices? How many acidic protons and how many basic sites do you have? What sort of groups do your pK measurements suggest?

Offline IS300bklyn

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Re: HELP with determine an unknown amino acid
« Reply #3 on: September 17, 2012, 04:50:03 PM »
I revised the graph but i still couldn't determine which amino acid is this?!??!?


Offline Borek

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Re: HELP with determine an unknown amino acid
« Reply #4 on: September 17, 2012, 05:10:44 PM »
Explaining what you did won't hurt.
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Offline IS300bklyn

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Re: HELP with determine an unknown amino acid
« Reply #5 on: September 17, 2012, 06:42:10 PM »
The blue curve is what i titrated the unknown amino acid with HCl and the green curve is NaOH.....next to it are the data points. problem is i couldnt make a good match on what kind of unknown amino acid i titrated with. Thanks

Offline Babcock_Hall

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Re: HELP with determine an unknown amino acid
« Reply #6 on: September 17, 2012, 07:18:13 PM »
Color me puzzled.  In looking at your first graphs, I am struck by the fact that the pKa values seem different from what a typical amino acid with a non-ionizing side chain would have.  For example, pK1 for alanine is 2.35, and pK2 is 9.87.  These values vary a little bit from one source to the next, but they don't vary by huge amounts.

Offline Borek

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Re: HELP with determine an unknown amino acid
« Reply #7 on: September 18, 2012, 04:01:52 AM »
There is something wrong with the NaOH curve. Are you sure pH meter was working OK?
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