[Nescafe]

Hi,

When it comes to studying drugs that are lethal to cells how would one know whether the mechanism by which the cells die as a result of treatment of this drug is Necrosis or apoptosis. I understand that one can monitor the markers of apoptosis v.s. necrosis but how would one know when to look for these markers? 30mins, 1hr, 2hr or the only way to know is to test at different time points and closely monitor the markers? Sounds super tedious if this is the way

Thanks in advance!

Nescafe.

[Arkcon]

Bam.  http://en.wikipedia.org/wiki/Necrosis

Cells that die due to necrosis do not usually send the same chemical signals to the immune system that cells undergoing apoptosis do. This prevents nearby phagocytes from locating and engulfing the dead cells, leading to a build-up of dead tissue and cell debris at or near the site of the cell death. For this reason, it is often necessary to remove necrotic tissue surgically, a process known as debridement.

Granted, the Wikipedia entry is talking about necrosis in the sense of a visible expanse of animal tissue.  However, the easiest answer is right in Wikipedia's header -- the chemical signals just aren't there.

[Nescafe]

Bam.  http://en.wikipedia.org/wiki/Necrosis

Cells that die due to necrosis do not usually send the same chemical signals to the immune system that cells undergoing apoptosis do. This prevents nearby phagocytes from locating and engulfing the dead cells, leading to a build-up of dead tissue and cell debris at or near the site of the cell death. For this reason, it is often necessary to remove necrotic tissue surgically, a process known as debridement.

Granted, the Wikipedia entry is talking about necrosis in the sense of a visible expanse of animal tissue.  However, the easiest answer is right in Wikipedia's header -- the chemical signals just aren't there.

That actually makes sense.

Thanks for the help. Here is a cookie!

Nescafe.

PS and by cookie I mean a snack