Is this as simple as I think??Im a biology student doing a module of analytical chemistry,Do I just plot the standard curve and read the moisturises sample from that?If so how do i calculate the %w/w of methyl paraben in the moisturiser.
"The analysis of methyl-p-hydroxybenzoate (methyl paraben) in cosmetic products is
performed routinely by reverse phase HPLC.
A 0.625g sample of moisturiser was shaken vigorously in 20 cm3
of methanol until well dispersed and the solution made up to 100 cm3 with methanol.
Standard solutions of methyl-p-hydroxybenzoate were made up as shown in the table
below. 20ul of each solution were injected into a HPLC and the following results
obtained:"
Peak Area (cm2)
Methyl
Standards(mg l-1) Paraben
2.0 0.52
4.0 1.01
6.0 1.54
8.0 2.05
10.0 2.60
Moisturiser 1.07
Plot an appropriate graph and use it to determine the %w/w of methyl paraben in the moisturiser.