Pipet 2.50 mL of the iron solution into five large test tubes. Add 2.50 mL of
the acetate buffer, 0.50 mL of the hydroxylamine hydrochloride solution and
mix. Pipet 0.50, 1.00, 1.50, 2.00 and 2.50 mL of the 5.00 x10-4 M
phenanthroline solution. Dilute to a total volume of 15 mL with water. Stand
for 10 min then measure absorbance.
*Iron solution conc= 5.0x10-4M
Repeat procedure except that you should use 5.00 mL of 1.50 x10-3 M
phenanthroline solution and 0.25, 0.50, 0.75, 1.00 and 1.25 mL of the iron
solution.
Plot absorbance versus iron concentration (M) and absorbance versus
phenanthroline concentration (M). From the slopes of these 2 graphs,
calculate stoichiometry.
I dont get why find the slope when the concentration here is constant in both graphs , or I just miss interpret this ? Help
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Topic: spectrometry (Read 2644 times)