[Lister]

I'm making media with 10.4% potassium phosphate dibasic, 5.4% potassium phosphate monobasic and 2% ammonium sulfate. I get a heavy precipitate. The recipe is taken from a publication so I'm assuming I should be able to get full solubility. Is there an sequence order to the solubilization that will prevent precipitation?
Thanks.

[Archer]

Does a precipitate form when you mix the stock solutions?

Can you provide the recipe that you used (weights and volumes) from your lab book, not the publication?

[Lister]

Oh, dear. Now I feel stupid. I was using Ferrous Ammonium Sulfate. I did not see the ferrous. I repeated with Ammonium Sulfate and its not problem! Lesson learnt - make sure you are seeing the whole label!
Thanks for responding. It prompted me to check the label.

[Archer]

Oh, dear. Now I feel stupid. I was using Ferrous Ammonium Sulfate. I did not see the ferrous. I repeated with Ammonium Sulfate and its not problem! Lesson learnt - make sure you are seeing the whole label!
Thanks for responding. It prompted me to check the label.

Don't feel stupid, I made a buffer for gel electrophoresis but didn't read that the solution I had made was a 100 x stock. Turned on the power, lots of bubbles and the gel melted we all make mistakes, stupid people don't learn from them.

All the best with your experiment.

[Lister]

Thank you! Its been over 10 years since my PhD and there seems to be an infinite number of mistakes to make. As you say, it keeps us learning.