I already know how to approach this problem.
1. ICE Table H-MOPS + OH
- MOPS
- + H
2O
initial------(x)----------(x)--------(48)-------NA
change ---(-x)---------(-x)-------(+x)-------NA
final-------(~0)--------(~0)------(48+x)----NA
2. Since we wan to make a pH = 6.85 buffer:pH = pKa + log(nb/na)
6.85 = (-log Ka) + log(___ /___)
6.85 = 7.19 + log(___ /___)
-0.34 = log(___ /___)
10
(-0.34) = log(___ /___)
0.457 = (___ /___)
Solve for x (which will be in mmol)
3. x mmol x (0.31 ml/mmol of H-MOPS) = AnswerThe problem is my table ISN'T set up right!
They gave me Mm of H-MOPS but I don't know what to use it for...
I need help setting up this table properly