[tribaggili]

I quote from a paper: "...a solution of CuSO4 (1 mM) and NaAsc (2 mM) in Tris buffer
(10 mM, pH 7. with 20% DMSO".

Does the molarities refer to the 80% volume of the Tris buffer or of the total volume (80% v Tris + 20% v DMSO)? For example, if I want to make 100 mL solution, I have to add 80 mL of 10 mM Tris buffer, or 80 mL of 12.5 mM Tris buffer (which is 10 mM in 100 mL).

Thanks!
Andrea

[Hunter2]

No for 100 ml  you add 1 mmol Tris and 20 ml DMSO .