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Topic: iodoacetamide stock solution?  (Read 5334 times)

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Offline Babcock_Hall

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iodoacetamide stock solution?
« on: May 20, 2014, 11:16:31 AM »
We do a good deal of protein modification in my lab, but we rarely work with iodoacetamide.  Can a stock solution in water be made, or is the rate of hydrolysis too fast?  I think that the amount we need is too small to be weighed out and added directly to the reaction.  We will be modifying a catalytic cysteine residue.
EDT
So far, everything I found suggests preparing an aqueous solution fresh at high concentration, such as 0.5 M (and presumably not trying to store it).  Besides hydrolysis, light is also a problem.
« Last Edit: May 20, 2014, 11:45:57 AM by Babcock_Hall »

Offline Babcock_Hall

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Re: iodoacetamide stock solution?
« Reply #1 on: May 27, 2014, 01:56:16 PM »
Some manufacturers sell this compound in small, pre-weighed aliquots.  This also suggests that single use is the way to go in cdertain applications.

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