[kamiyu]

How do you guys add the internal standard ferrocene??

Some add TOGETHER with the sample but I add it separately. I think both of them work fine, but there is someone who says adding separately is wrong....

[Hunter2]

Internal means you have to add. Otherwise its an external standard.

[kamiyu]

But I think both ways work. right?

[Hunter2]

I dont know your analysis, so cannot give a recommandation to it.

[ironnica]

Can you please expand on what you do/order of addition?

i.e. I work in electrocatalysis. I cannot add ferrocene until the final step, to make sure ferrocene does not affect catalysis or act in conjunction with my system.

[kamiyu]

I am doing Cyclic Voltammetry where the ferrocene is the standard whose HOMO is known to be -4.8eV.

My protocol is (actually taught by some senior in former school): after measuring the sample without ferrocene. Then run a final run of ferrocene and the E1/2 of this run will be the reference point, i.e. -4.8eV.

But I was told I had to add ferrocene together with sample as "internal standard". I am NOT saying that adding ferrocene with sample is wrong, but running separately is also fine and this is NOT wrong