[kelvem]

Hi,

I am master student in agriculture engineering. Proline amino acid is so important for plants. It is the indicator that plant is in stress or not. So I need to proline extraction and I found a video about it but it is not enough to get the logic of the analyze.

Video link :http://youtu.be/mvpLNT_bAPs

So what is the red and white things in this video and what is the method ?

Thanks..

[OrgXemProf]

 You asked about the "red stuff"; the following information appears online (Reference: http://www.scientistsolutions.com/a7356-protocol-free+proline+estimation+in+plants.aspx)

Introduction

Proline (abbreviated as Pro or P) is a α-amino acid, one of the twenty DNA-encoded amino acids found in proteins. It is unique among the 20 protein-forming amino acids in that the α-amino group is secondary. The more common L form has S stereochemistry.

In plants proline is synthesized from glutamic acid through a pathway catalyzed by pyrroline-5-carboxylate synthetase (1) and pyrroline-5-carboxylate reductase.

Its accumulation under various abiotic stresses (heat, cold, drought, moisture and salinity) in important crop plants considered as a tolerance mechanism. It is suggested to act as an osmolyte/compatible as well as a source of nitrogen during recovery from stress. Compatible solutes act a as chemical chaperone, which protects proteins during various abiotic stresses. 

Proline estimation is based on the formation of brick red colored praline-ninhydrin complex in acidic medium. This complex is soluble in toluene and thus can be separated from aqueous phase. This ensures that there is no interference with other amino acids, which also form blue colored complex with ninhydrin. The toluene soluble brick-red colored complex absorbs at 520 nm.

* * *

The Scientist Solutions article goes on to describe an extraction procedure.

The following primary literature reference appears at the end of this article:

Bates, L.S., Waldran, R.P. and Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant Soil 39: 205-208.

[kelvem]

Thank you so much for your reply, It was so clear and informative..