[Insegnami]

Dear members,

I have a question about dissolving Iodo-deoxyuridine (IdU), C9H11IN2O5,  in acidified water (with HCl).
Idu is used for labeling proliferating cells, because it can be incorporated into the DNA-strand. And I want it to dissolve it in acidified water for my experiments.

In the product description it says:  Solubility method: 50mg/ml in 1 N ammonium hydroxide. Although most colleagues dissolve it in demi-water.
How can I find out if I can dilute it in acidified water (pH 2,5-3)? It is really important that the IdU is chemically stable, so the cells can recognize it.

Thanks in advance.

[Arkcon]

Well, your question is a little confusing.  Your colleagues can dissolve it in dist water (I'm guessing, I don't recognize demi-water.)  You have a method that specifies a dilute solution of base.  You intend to use it on living cells, which I assume prefer to be in a carefully buffered neutral pH environment.  Where did you get the idea to use a low pH?  Where did you read it?  That source may give insights into stability and bio-availability at low pH.

[Insegnami]

Yeah sorry, I left that part out.
Demi water = dist water.
I intend to use it in drinking water for mice. It has to be acidified to prevent bacteria and fungi growth in the drinking water. We need to dissolve 1 mg/mL in acidified water (max pH of 4).