[ikakurnia]

how to recover my nitro eugenol compound from saturated silica in chromatography column? i was use liquid chromatography.
thank you

[k1mng]

Hi ikakurnia,

If you were following a prep it should have some information on recovering the compound. Have you tried flushing through the compound with any strongly eluting solvent systems yet?

[TheUnassuming]

What solvent system are you using?

[ikakurnia]

i have flush with n hexane and DCM, but the silica was saturated, so my compound doesn't good separated. The compound should be dissolve in DCM, but it dissolve to in hexane to. I was wrong when adding slurry of silica. the slurry of silica i use isn't notice the ratio between gram of silica and gram of sample. Sample that containing nitro eugenol was 4.5 g, but i use silica measly, so the silica was going saturated.

[ikakurnia]

i use acetonitril as a solvent in sample containing nitro eugneol, but it measly because it has evaporate.

[TheUnassuming]

Ok, well your solvent system is still relatively nonpolar so you have a lot of room to play.  Try increasing the polarity of your mobile phase up to 15% MeOH in EtoAc.  It should clean anything off the column without getting appreciable silica contamination off the column. 
Remember that just because something is soluble in a solvent doesn't mean it will come off a column well using just that solvent.  DCM is a great example of this.