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Topic: four parametric logistic curne fit in GraphPad Prism  (Read 4265 times)

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Offline lab2015

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four parametric logistic curne fit in GraphPad Prism
« on: September 08, 2015, 08:11:52 AM »
I need to do a 4PL in graphpad prism. I am new to this software and trying to learn how to do this. I am doing the following steps:
from the welcome message I choose Xy then enter 2 replicates values in side-by-side subcolumns then create
I enter my data: concentrations of standards and the corresponding readings in duplicate and the samples readings in duplicate as well
from the analysis options I choose: interpolate a standard curve and then choose Sigmoid, 4PL, x is log(concentration)

is this correct?

Please help
regards

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