November 25, 2024, 04:31:28 PM
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Topic: Help with Average Charge Question On Ionizable Functional Group of a Protein  (Read 2235 times)

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Offline student12393

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So the question is as follows:

A hypothetical protein with a single ionizable functional group takes on a charge of -1 when deprotonated. Find the equation for the average charge Z on the molecules as a function of pH and pKA of this functional ionizable group.

So the only equation I know of in regards to charge "Z" is the the Ionic Strength formula which is I = 1/2 Ī£(i) ciZi^2. I'm not sure how to relate this at all with pH or pKA, and have had no luck when trying to come with something using the Hendersonā€“Hasselbalch equation.

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