[Jefke]

Hallo all,

I saw a post about a competitive inhibitor used in a yeast two hybrid system. Now I had to study this system during my biology classes, but never realised it back then.

If you use a yeast two hybrid system they always say you can use 3-AT, the competitive inhibitor for the enzym you produce with your reporter gene (his3), but would it be possible to just use another product that is a competitive inhibitor of another gene/enzym in the same pathway ?
Or is this too difficult/different from using a direct competitive inhibitor?

Or, the other way around, just use a different enzym/gene as a reporter (but still use the same prodcut: 3-AT?

Any ideas?