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Topic: UV-VIS measurement  (Read 2469 times)

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Offline Spacenova

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UV-VIS measurement
« on: December 21, 2015, 02:55:53 AM »
I need to measure UV assay of a drug substance. I measure standard material 3 times and chcek the calibration linear with a control solution. Then I create average of standards. Should I calculate only from 3 standards or calculate with control as well?

 Thank you.
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Offline ttn1979

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Re: UV-VIS measurement
« Reply #1 on: January 09, 2016, 04:51:22 AM »
You should calculate from 3 times of measurement
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Offline Borek

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Re: UV-VIS measurement
« Reply #2 on: January 09, 2016, 05:30:40 AM »
For me the question is about as ambiguous as the answer.
« Last Edit: January 09, 2016, 08:37:36 AM by Borek »
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Offline Arkcon

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Re: UV-VIS measurement
« Reply #3 on: January 09, 2016, 07:41:53 AM »
You will have to define what you mean by control better.  Do you mean a separate standard prepared as a check standard, or do you mean bracketing standards, the same prep as beginning standards, that are run after the samples?
« Last Edit: January 10, 2016, 07:34:48 AM by Arkcon »
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