[littlejoker]

Can a carboxypeptidase cleave the peptide bond at C-terminal of an amidated peptide?

[AWK]

https://en.wiki2.org/wiki/Carboxypeptidase+Newton
https://en.wiki2.org/wiki/Dipeptidyl_carboxypeptidase+Newton

[littlejoker]

Thank you.
So it cannot cleave blocked peptides, right?

[AWK]

Peptides may be blocked in different ways. Name of enzyme itself informs you which group should be free,

[Babcock_Hall]

At the active site of carboxypeptidase A is an arginine residue that is positioned to bind to the carboxylate residue of the C-terminal amino acid residue.  Without the charge, my prediction is that the rate would be much slower, but one would have to answer this question empirically for each type of carboxypeptidase.  One place to start a search for such information might be BRENDA.