Hello!
I am trying to follow protocol for TLC of glucose. Mobile phase contains CH3COOH:NH4OH 0.35M: Acetonitrile = 1:1:8.
It was too polar for glucose and I tried to modify it to CH3COOH:NH4OH 0.35M: Acetonitrile: Hexane = 1:1:2:6. But it didn't help.
How can I decrease polarity?
From other solvents I have EtAc; EtOH; ProOH; Hexane and Chloroform.
Thanks,
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