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Topic: TLC of glucose  (Read 1841 times)

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Offline Flame

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TLC of glucose
« on: September 05, 2018, 02:19:48 AM »
Hello!
I am trying to follow protocol for TLC of glucose. Mobile phase contains CH3COOH:NH4OH 0.35M: Acetonitrile = 1:1:8.
It was too polar for glucose and I tried to modify it to CH3COOH:NH4OH 0.35M: Acetonitrile: Hexane = 1:1:2:6. But it didn't help.

How can I decrease polarity?
From other solvents I have EtAc; EtOH; ProOH; Hexane and Chloroform.


Thanks,
Flame


Offline Babcock_Hall

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Re: TLC of glucose
« Reply #1 on: September 05, 2018, 08:30:08 AM »
Egon Stahl's venerable book on TLC has a chapter on carbohydrates IIRC.  Ten hours in the lab can save you an hour in the library.

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