[Sgriger534]

So I am currently working on updating some analytical procedures where I work and one is a fluorometric riboflavin assay

My question is that the procedure uses 0.2n Hcl to dissolve the sample.  However every other similar procedure I can find (e.g. USP, AOAC) uses 0.2n acetic acid.
Is there any benefit to using one acid over the other?

[Babcock_Hall]

I don't know the answer, but there is going to be a large pH difference, and I would at least consider how stable riboflavin is at the lower pH value.

[Sgriger534]

As in the other methods,  the ph is adjusted (although I can't remember the value currently) but it falls within the same range as the methods using acetic acid