[Biotech78]

Hi

Can anybody recommend a good paper which I can follow for EDC/NHS coupling of Ab to -COOH-NP. I know there are many out there but all the papers I see use huge quantities of Ab which I don't think I can afford when using a commercially available Ab.
Just haven't been able to get my head around what should be the ratio of NP and Ab

[wildfyr]

Try to work out the number of active sites on each nanoparticle. That should get you within an order of magnitude or two of what you need