[txxplc110]

Hello!
I have a question about HPLC chromatograms. I got two chromatograms, each one with different signal/wavelength (290nm and 320nm) and I got two peaks of compounds that I wanted to analyze, but the peaks are not on the same chromatogram. My question is, can I read peak resolution of two peaks from two different chromatograms, or not? It doesn’t make any sense for me, but it's in my instruction. I can’t find any information about that. I would be most grateful if you would look into this matter.

[shchavel]

Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.

[txxplc110]

Hello!
I am not a HPLS specialist, but i think, that it's uncorrect. You must get this peaks in 1 expirement/chromatogramm to correct compare.

It turned out to be correct. It was in the same injection but at a different wavelength and an expert told me that I can calculate it.