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Topic: How can I hydrolyze whey protein isolate at home?  (Read 1009 times)

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Offline Mb1038

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How can I hydrolyze whey protein isolate at home?
« on: June 16, 2020, 11:17:50 AM »
I have no chemistry experience at all.
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Offline AWK

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Re: How can I hydrolyze whey protein isolate at home?
« Reply #1 on: June 16, 2020, 01:09:26 PM »
If you don't have chemical experience then you will also have a problem with protein isolation.
Proteins are hydrolyzed by heating with 1 M HCl in a sealed glass ampoule immersed in boiling water for several hours (~10 or more).
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Offline Babcock_Hall

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Re: How can I hydrolyze whey protein isolate at home?
« Reply #2 on: June 16, 2020, 01:55:12 PM »
Ideally, the acid should be free of oxidizing impurities, and the acid/protein mixture is put through cycles of freezing, applying a vacuum, and thawing to remove oxygen.
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